Efecto Estrogénico del extracto Hidroalcóholico de alfalfa (Medicago Sativa) en Ratas Albinas Ovariectomizadas
Date
2010
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Nacional Mayor de San Marcos
Abstract
Objetivo: Demostrar las propiedades estrogénicas del extracto hidroalcohólico de la especie Medicago sativa L (alfalfa) en ratas albinas ovariectomizadas (OVX). Método: Se emplearon 48 ratas albinas Sprague Dawley hembras de 200 a 250 g. de 8 semanas de edad de las cuales 40 fueron sometidas a extirpación quirúrgica de ambos ovarios (OVX) siguiendo la técnica de ovariectomía bilateral por el flanco. Después del post-operatorio las ratas se dividieron en seis grupos y se suministró el extracto hidroalcohólico de Medicago sativa (alfalfa) bajo el siguiente esquema de trabajo: Grupo 1:Control Negativo (OVX), vehículo, VO,2mL/kg; Grupo2: Control Positivo (OVX) ,Estradiol, SC, 3ug/kg; Grupo 3: (OVX)Extracto Alfalfa ,VO, 100 mg/kg; Grupo 4: (OVX),Extracto Alfalfa, VO, 500 mg/kg; Grupo 5: (OVX), Extracto Alfalfa, VO, 1000 mg/kg; Grupo 6: (No OVX),Control del procedimiento quirúrgico. El tratamiento duró 14 días.
Los parámetros evaluados fueron peso del útero, peso corporal, cambios del ciclo estral mediante frotis vaginal y análisis del perfil hormonal. Resultados: Se observó aumento en el peso de útero en las dosis de 500mg/kg y 1000mg/kg.; además de la presencia de alcaloides, flavonoides y saponinas en el extracto hidroalcohólico de alfalfa en cantidad regular. En la técnica quirúrgica empleada la combinación anestésica xilazina (2mg/kg), ketamina (40mg/kg) indujo un plano quirúrgico óptimo (plano 2), sin complicaciones en el post-operatorio ni la muerte de los animales.
Conclusiones: El extracto hidroalcohólico de Medicago sativa L (alfalfa) causa un efecto estrogénico al incrementar el peso de útero en ratas OVX en las dosis de 500mg/kg y 1000mg/kg. Además contiene saponinas en cantidad regular. La técnica quirúrgica ovariectomía bilateral por el flanco es un método práctico, confiable y menos traumático para los animales que permite realizar investigaciones sobre defiencia de estrógenos.
Objective: Demonstrate the estrogenic properties of hydroalcoholic extract of the species Medicago sativa L (alfalfa) in albino rats ovariectomized (OVX). Methods: We used 48 Sprague Dawley female albino rats of 200 to 250 g. 8 weeks of age, of which 40 underwent surgical removal of both ovaries (OVX) by means of bilateral ovariectomy on the flank. After post-operative rats were divided into six groups and provided the hydroalcoholic extract of Medicago sativa (alfalfa) under the following work schedule: Group 1: Negative Control (OVX), vehicle, VO 2 ml / kg, Group 2: Positive Control (OVX), estradiol, SC, 3ug/kg; Group 3 (OVX) Alfalfa extract, VO, 100 mg / kg, Group 4 (OVX), Alfalfa extract, VO, 500 mg / kg, Group 5: (OVX), Alfalfa extract, VO, 1000 mg / kg, Group 6: (No OVX), Control of the surgical procedure. The treatment lasted 14 days. The outcome measures were uterine weight, body weight, estrous cycle changes by vaginal smear and hormonal profile analysis. Results: The increase in uterine weight at doses of 500mg/kg and 1000mg/kg., Besides the presence of alkaloids, flavonoids and saponins in the hydroalcoholic extract of alfalfa in regular amount. In the surgical technique used anesthetic combination xylazine (2mg/kg), ketamine (40mg/kg) induced an optimal surgical plane (plane 2), without complications or postoperative death of animals. Conclusions: The hydroalcoholic extract of Medicago sativa L (alfalfa) causes an oestrogenic effect by increasing the uterus weight in OVX rats at doses of 500mg/kg and 1000mg/kg. It also contains saponins in regular amount. The surgical technique for bilateral ovariectomy flank is a practical, reliable and less traumatic for the animals that can do research on estrogen deficiency.
Objective: Demonstrate the estrogenic properties of hydroalcoholic extract of the species Medicago sativa L (alfalfa) in albino rats ovariectomized (OVX). Methods: We used 48 Sprague Dawley female albino rats of 200 to 250 g. 8 weeks of age, of which 40 underwent surgical removal of both ovaries (OVX) by means of bilateral ovariectomy on the flank. After post-operative rats were divided into six groups and provided the hydroalcoholic extract of Medicago sativa (alfalfa) under the following work schedule: Group 1: Negative Control (OVX), vehicle, VO 2 ml / kg, Group 2: Positive Control (OVX), estradiol, SC, 3ug/kg; Group 3 (OVX) Alfalfa extract, VO, 100 mg / kg, Group 4 (OVX), Alfalfa extract, VO, 500 mg / kg, Group 5: (OVX), Alfalfa extract, VO, 1000 mg / kg, Group 6: (No OVX), Control of the surgical procedure. The treatment lasted 14 days. The outcome measures were uterine weight, body weight, estrous cycle changes by vaginal smear and hormonal profile analysis. Results: The increase in uterine weight at doses of 500mg/kg and 1000mg/kg., Besides the presence of alkaloids, flavonoids and saponins in the hydroalcoholic extract of alfalfa in regular amount. In the surgical technique used anesthetic combination xylazine (2mg/kg), ketamine (40mg/kg) induced an optimal surgical plane (plane 2), without complications or postoperative death of animals. Conclusions: The hydroalcoholic extract of Medicago sativa L (alfalfa) causes an oestrogenic effect by increasing the uterus weight in OVX rats at doses of 500mg/kg and 1000mg/kg. It also contains saponins in regular amount. The surgical technique for bilateral ovariectomy flank is a practical, reliable and less traumatic for the animals that can do research on estrogen deficiency.
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Keywords
Alfalfa - Uso terapéutico, Ratas como animales de laboratorio, Plantas medicinales - Perú