Browsing by Author "Poma Acevedo, Astrid Gloria"

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    A transient increase in MHC-IIlow monocytes after experimental infection with Avibacterium paragallinarum (serovar B-1) in SPF chickens
    (Veterinary Research, 2020-09-25) Alvarez, Karla Lucía F.; Poma Acevedo, Astrid Gloria; Fernández-Díaz, Manolo
    Infectious coryza (IC), an upper respiratory tract disease affecting chickens, is caused by Avibacterium paragallinarum. The clinical manifestations of IC include nasal discharge, facial swelling, and lacrimation. This acute disease results in high morbidity and low mortality, while the course of the disease is prolonged and mortality rates are increased in cases with secondary infections. Studies regarding the immune response in infected chickens are scarce, and the local immune response is the focal point of investigation. However, a large body of work has demonstrated that severe infections can impact the systemic immune response. The objective of this study was to evaluate the systemic effects of Avibacterium paragallinarum (serovar B-1) infection on immune cells in specific pathogen-free (SPF) chickens. The current study revealed the presence of a transient circulating monocyte population endowed with high phagocytic ability and clear downregulation of major histocompatibility complex class II (MHC-II) surface expression. In human and mouse studies, this monocyte population (identified as tolerant monocytes) has been correlated with a dysfunctional immune response, increasing the risk of secondary infections and mortality. Consistent with this dysfunctional immune response, we demonstrate that B cells from infected chickens produced fewer antibodies than those from control chickens. Moreover, T cells isolated from the peripheral blood of infected chickens had a lower ability to proliferate in response to concanavalin A than those isolated from control chickens. These findings could be related to the severe clinical signs observed in complicated IC caused by the presence of secondary infections.
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    Desarrollo de un protocolo de detección de células de pollo (línea celular DF-1) infectadas por el Herpes Virus de Pavo (HVT) mediante citometría de flujo
    (Universidad Nacional Mayor de San Marcos, 2022) Poma Acevedo, Astrid Gloria; Valdivia Cuya, Martha Esther
    Desarrolla un protocolo que permita detectar células infectadas en 24 horas, a través de citometría de Flujo. El protocolo desarrollado, basado en la detección intracelular de proteínas virales mediante el uso del antisuero MDV, ha mostrado ser específico para células infectadas y ha sido capaz de detectar el incremento de éstas durante el periodo de cultivo. Con la finalidad de confirmar que el protocolo no esté subestimando o sobrestimando el porcentaje de células infectadas, células DF-1 fueron infectadas con un virus recombinante (rHVT) que expresaba la proteína verde fluorescente (HVT-GFP). Posteriormente evaluamos el porcentaje de células infectadas utilizando el protocolo desarrollado y paralelamente evaluamos el porcentaje detectado a través de la expresión de GFP. Mediante la correlación de Spearman observamos una alta correlación (r= 0.93; p=0.011) entre ambos resultados. En conclusión, el protocolo desarrollado es capaz de detectar proteínas virales intracelulares durante el proceso de infección.